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Reagents & materials

Semen Evaluation - Eosin-Nigrosin (Breeding Soundness) - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Semen (E-N)

Veterinary & animal health · Parasitology, breeding and related applications

Eosin–nigrosin; Hancock-type and Giemsa semen methods

Selected sperm morphology and viability assessments

Reagents & stains

ItemProcure
Eosin-nigrosin vitality setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Separate Hancock-type morphology reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
GiemsaPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Matched diluent/mountant if neededPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional species-validated semen vitalityPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Morphology setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Slides/coverslips as requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
MicrotubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield/phase-contrast microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Species-appropriate fixed morphology referencesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Validated vitality referencePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Framework): SFT breeding-soundness guidance is species-specific. Catalog verification does not authenticate every dye as interchangeable or establish a universal WOAH/CSS staining requirement.

Procurement note: Hancock/Giemsa morphology differs from vitality. SFT scope is not a universal dye recipe for all centres.

Sources:G058 Society for Theriogenology

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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