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Reagents & materials

Gram Stain - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Gram

Human (Clinical) · Bacteriology and microbiology

Gram stain including Sandiford modification

Bacterial morphology and Gram reaction in clinical specimens and cultures

Reagents & stains

ItemProcure
Crystal violetPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
IodinePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
DecolorizerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CounterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Separate pyronin Y/malachite-green counterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Method-compatible fixativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Immersion oilPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional ready-made Gram setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sandiford counterstain is a separate variantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Glass slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Labelled slide boxesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Transfer pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Stain jars/racksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Coverslips if specifiedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscopePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining stationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Validated fixed positivePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Negative control slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): UK SMI TP39 sections 6.2 and 6.6. Report morphology and specimen context; staining is not species identification.

Procurement note: TP39 sections 6.2/6.6 document reagent identities. Select one validated counterstain system; standard supplies are planning items, not a formulation.

Sources:G009 UK Health Security Agency and Royal College of Pathologists

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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