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Reagents & materials

SYBR Gold, SYBR Green I, propidium iodide or ethidium bromide - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

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Regulatory toxicology & chemical safety laboratories · General

SYBR Gold, SYBR Green I, propidium iodide or ethidium bromide

DNA damage measurement

Reagents & stains

ItemProcure
SYBR GoldPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
SYBR Green IPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PI or ethidium bromide. Low-melting agarosePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Validated lysis solutionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Electrophoresis/unwindingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Neutralisation buffersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Wash solutionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional comet-assay reagent kit. Documented example: R&D Systems CometAssay kit with LMAgarose, lysis solution, EDTA and CometSlides. Check whether the chosen fluorescent dye is separately suppliedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Prepared cell samplesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Agarose-compatible comet slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Slide-processing vesselsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Electrophoresis tankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Power supplyPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Controlled-temperature equipmentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Fluorescence microscope/cameraPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Validated comet-image analysis softwarePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Qualified assay-control cell preparations with defined DNA damagePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Negative controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Gel/stain blanksPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): OECD TG 489 provides the in vivo mammalian alkaline comet test. State the validated dye and image analysis endpoint; comet staining is not an apoptosis specific assay.

Procurement note: Different DNA stains are alternatives. Kit hardware, fluorescent stain and control cells may be separate purchases. Regulatory implementation follows TG 489 and the validated system.

Sources:G149 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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