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Methylene blue formation - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Methylene blue formation

Environmental water wastewater & soil testing · General

Methylene blue formation

Sulfide determination in water

Reagents & stains

ItemProcure
N-dimethyl-p-phenylenediamine colour reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Ferric-chloride reagentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Specified acidic mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sulfide standardPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sample-preservation reagents where requiredPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional method-compatible methylene-blue sulfide reagent setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Matched cells/cuvettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Closed sample vesselsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible chemical-waste containersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Spectrophotometer/colorimeterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calibrated pipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Chemical ventilationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Sulfide calibration/check standardPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent blankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Matrix controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): EPA 376.2 is a separate colorimetric sulfide method. It must not be confused with MBAS surfactant testing despite the shared colour name.

Procurement note: Methylene blue is generated by the colour reaction. Purchasing methylene-blue stain alone does not supply the sulfide assay.

Sources:G131 US EPA

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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