Reagents & materials
Gram and Neisser stains - Reagents & Materials
What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).
Environmental water wastewater & soil testing · General
Gram and Neisser stains
Activated sludge filament characterisation
Reagents & stains
| Item | Amount per test | Procure |
|---|---|---|
| Crystal violetPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Gram iodinePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Method-matched decolorizerPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Safranin or specified counterstainPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Reagent waterPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Immersion oil. Neisser: authenticated Neisser primary solutionsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Chrysoidin or Bismarck-brown counterstain specified by the variantPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Kits
| Item | Amount per test | Procure |
|---|---|---|
| Optional separate GramPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Neisser staining setsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Consumables & materials
| Item | Amount per test | Procure |
|---|---|---|
| Clean glass slidesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| CoverslipsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Disposable pipettes or tipsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Labelled specimen tubesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Absorbent paperPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Equipment
| Item | Amount per test | Procure |
|---|---|---|
| Brightfield microscopePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Calibrated pipettesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Staining rackPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Jars. Phase-contrast optics for associated unstained sludge examinationPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Controls & reference materials
| Item | Amount per test | Procure |
|---|---|---|
| Fixed filament/reference slidesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Reagent blankPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Official basis (Framework): Operational microscopy under a documented sludge identification scheme. Eikelboom keys and practitioner manuals are not universal discharge compliance standards.
Procurement note: Morphological sludge characterisation is locally validated. The exact Neisser variant and reference filament atlas determine interpretation.
Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.