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Reagents & materials

Neutral red uptake and fluorescein leakage - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to NRU & Fluorescein Leakage

Cosmetics & personal care · General

Neutral red uptake and fluorescein leakage

Phototoxicity or severe ocular hazard endpoints

Reagents & stains

ItemProcure
Neutral redPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible assay medium/bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Ethanol/acetic-acid dye extractant. Fluorescein leakage: sodium fluoresceinPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Calcium-containingPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Phenol-red-free HBSSPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional NRU reagent kit. Fluorescein-leakage components are selected to the validated barrier-test system. These are separate assaysPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Qualified 3T3 cell preparationsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Assay plates. Leakage: qualified MDCK barrier preparationsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Membrane insertsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Receiving plates. Sterile tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Controlled irradiation system with radiometerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbance plate readerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Cell-assay equipment. Leakage: fluorimeter/fluorescence plate readerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Barrier-assay equipmentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
NRU light/darkPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Positive/negative controls. Leakage negativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PositivePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Maximum-leakagePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Blank controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): These are different tests: TG 432 addresses phototoxicity and TG 460 selected severe ocular hazards. Their endpoints and applicability must remain separate.

Procurement note: Do not combine the two reagent lists into one assay. Phototoxicity and ocular barrier leakage have distinct test systems and controls.

Sources:G121 OECDG122 OECD

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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