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Reagents & materials

Gram stain - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Gram stain

Pharmaceuticals biopharma cell therapies & medical devices · General

Gram stain

Characterisation of recovered contamination or environmental isolates

Reagents & stains

ItemProcure
Crystal violetPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Gram iodinePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Validated alcohol/acetone decolorizerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Safranin or the selected counterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent waterPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Immersion oil. Fixative if required by the selected methodPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional ready-to-use Gram stain set. Check whether counterstainPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
FixativePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
ControlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Oil are includedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Clean glass slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Slide labelsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Staining rack or jarsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Disposable applicatorsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Absorbent materialPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Brightfield microscope with oil-immersion capabilityPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Slide holderPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Appropriate sample-handling facilitiesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Fixed Gram-positivePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Gram-negative control smears with known reactionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): FDA pharmaceutical microbiology guidance and GMP support organism investigation. Gram staining is an adjunct; a negative smear is not a sterility result.

Procurement note: Choose one validated Gram variant. Gram staining does not establish pharmaceutical sterility.

Sources:G108 US FDAG008 European Commission

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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