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Reagents & materials

Osmium tetroxide, ruthenium tetroxide and phosphotungstic acid - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to OsO4 / RuO4 / PTA (EM)

Materials science electronics & nondestructive testing · General

Osmium tetroxide, ruthenium tetroxide and phosphotungstic acid

Polymer electron-microscopy contrast

Reagents & stains

ItemProcure
Supplied osmium tetroxide solutionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Supplied ruthenium tetroxide solutionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
OR phosphotungstic acid reagent. Carrier/embedding medium must be selected for the polymer and routePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional sealed EM contrast reagent ampoulesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
No combined Os/Ru/PTA kit or on-site generation is assumedPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Polymer blocks/sectionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible sealed staining vesselsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TEM grids or SEM mounts as appropriatePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
KnivesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Embedding suppliesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Ultramicrotome or other validated section preparationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TEM or suitable SEMPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dedicated extracted enclosure for volatile metal oxidantsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Unstained sectionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Known polymer phase referencePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Carrier/embedding blankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Imaging calibrationPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Research): Research or material-specific contrast methods. No universal official stain protocol is assigned.

Procurement note: OsO4/RuO4 and PTA are distinct alternatives. Volatile oxidants require compatible sealed handling, chemical protection and dedicated metal-containing waste; supplier guidance does not validate every polymer.

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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