Reagents & materials
Direct blue, acid fuchsin and chlorazol black E - Reagents & Materials
What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).
Plant health seed testing & plant research · General
Direct blue, acid fuchsin and chlorazol black E
Mycorrhizal root colonisation
Reagents & stains
| Item | Amount per test | Procure |
|---|---|---|
| Direct blue, acid fuchsin or chlorazol black E. KOH clearing reagent, HCl, glycerol, lactic acid and waterPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Bleaching reagent only if the validated method requires itPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Kits
| Item | Amount per test | Procure |
|---|---|---|
| Individual ready-to-use mycorrhizal stain/clearing reagents may be usedPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| No kit requiredPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Consumables & materials
| Item | Amount per test | Procure |
|---|---|---|
| Fine-perforation tissue cassettesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Root containersPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| SlidesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| CoverslipsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| ForcepsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Equipment
| Item | Amount per test | Procure |
|---|---|---|
| Brightfield/stereo microscopePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Laboratory hotplate or approved heating apparatusPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Chemical extraction ventilationPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Controls & reference materials
| Item | Amount per test | Procure |
|---|---|---|
| Reference colonised root slidePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Noncolonised root materialPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Official basis (Research): INVAM documents these stains for root preparations. This is an institutional research procedure; the scoring approach must be stated separately.
Procurement note: Stains are alternatives. Listed constituents follow the INVAM research method; laboratory batch and matrix suitability still require verification.
Sources:G062 INVAM University of Kansas
Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.