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ELISA colour substrates including pNPP - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to ELISA Substrates (pNPP)

Plant health seed testing & plant research · General

ELISA colour substrates including pNPP

Plant bacterial and viral serology

Reagents & stains

ItemProcure
Target capture antibodyPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Target detection antibody/enzyme conjugatePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Extraction bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Wash bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Conjugate diluentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PNPP substratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Substrate buffer for alkaline-phosphatase detectionPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional complete target-specific DAS-ELISA kit. Agdia PathoScreen is a documented examplePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent-only sets may omit buffers/substratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Microtitre platesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate sealsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Extraction bags or tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent reservoirsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Plate reader matched to the substratePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plate washer or wash dispenserPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Plant-tissue homogeniserPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Kit positive controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Healthy-matrix negative controlPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Substrate blankPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Method): PM 7/101 covers bacterial ELISA and PM 7/125 viral ELISA. They are not blanket standards for every tissue blot or colour substrate.

Procurement note: Components depend on target and ELISA format. Do not substitute a clinical serum ALP kit for a plant ELISA detection system.

Sources:G061 EPPO

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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