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Reagents & materials

Direct Immunofluorescence - Skin - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to Skin DIF

Human (Clinical) · Histology histopathology and neuropathology

Direct skin immunofluorescence

Autoimmune blistering disease and selected connective tissue disorders

Reagents & stains

ItemProcure
Direct-IF immunoglobulin/complement conjugatesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Wash bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Fluorescence mountantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Accepted transport/freezing mediumPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Optional direct tissue-IF panel or validated individual conjugatesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Assay substrate slides or prepared sectionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dilution tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Humid chamberPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Cryostat/adhesive slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorescence microscope with matched filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent storage per IFUPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Cryostat accessPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Validated tissue-IF positive/negative reference sectionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Conjugate controlsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): Use disease appropriate biopsy site, transport and antibody panel. EADV guidance is indication specific; the lupus band test is not a standalone general lupus diagnosis.

Procurement note: Confirm transport/fixation with receiving laboratory; routine formalin is not an automatic substitute. Direct tissue IF differs from serum IFA.

Sources:G034 European Academy of Dermatology and Venereology

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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