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Reagents & materials

ANCA Immunofluorescence (IIF) - Reagents & Materials

What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).

Back to ANCA IIF

Human (Clinical) · Bacteriology and microbiology

Indirect immunofluorescence serology

Rickettsial and selected other bacterial infections

Reagents & stains

ItemProcure
Purchased fixed antigen substratesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Class-specific anti-human fluorescent conjugatePer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Sample diluentPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Wash bufferPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Compatible mountantPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Kits

ItemProcure
Target-specific IFA kit or validated complete component setPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Consumables & materials

ItemProcure
Assay substrate slides or prepared sectionsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
CoverslipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Dilution tubesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Pipette tipsPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Humid chamberPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Equipment

ItemProcure
Fluorescence microscope with matched filtersPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
PipettesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
TimerPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Reagent storage per IFUPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Controls & reference materials

ItemProcure
Assay-specific positive/negative control sera or fixed reference slidesPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site
Fluorescence system QCPer validated method / manufacturer IFUMerckRecommended · Official manufacturer site

Official basis (Guidance): CDC tickborne guidance supports paired serum IFA for relevant rickettsial infections. Coxiella, Bartonella, Leptospira, Borrelia and Brucella need their own test algorithm; no universal titre applies.

Procurement note: Pathogen, substrate and Ig class determine the kit. No universal conjugate or self-prepared live antigen material is specified.

Sources:G013 CDC

Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.

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