Reagents & materials
Ninhydrin, bromocresol green, 2,4-DNPH, FeCl3, Dragendorff and Ehrlich reagents - Reagents & Materials
What you need to perform one test: reagents, kits, consumables, equipment and quality controls, with their official basis. Quantities follow the validated method or the manufacturer's instructions for use (IFU).
Chemical & analytical laboratories · General
Ninhydrin, bromocresol green, 2,4-DNPH, FeCl3, Dragendorff and Ehrlich reagents
Functional group detection on TLC
Reagents & stains
| Item | Amount per test | Procure |
|---|---|---|
| NinhydrinPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Bromocresol green indicatorPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Stabilized/wetted 2Per validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| 4-DNPH reagentPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Ferric chloridePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Dragendorff bismuth/iodide reagentPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Ehrlich p-dimethylaminobenzaldehyde reagent. Each needs its specified acid/basePer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Aqueous or organic carrierPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Kits
| Item | Amount per test | Procure |
|---|---|---|
| Optional prepared functional-group TLC detection reagent setPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Select only the required reactionPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Consumables & materials
| Item | Amount per test | Procure |
|---|---|---|
| Specified TLC platesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Spotters/tipsPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Reagent bottlesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Dipping or spray accessoriesPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Solvent-resistant traysPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Equipment
| Item | Amount per test | Procure |
|---|---|---|
| TLC developing chamberPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Extraction for solvent/reagent handlingPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Visible-light documentationPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Heater only if selected method requires itPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Controls & reference materials
| Item | Amount per test | Procure |
|---|---|---|
| Known positive functional-group standardPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Negative standardPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| SolventPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
| Reagent blanksPer validated method / manufacturer IFU | Per validated method / manufacturer IFU | MerckMerck (Sigma-Aldrich)Recommended · Official manufacturer site |
Official basis (Framework): Use the analyte-specific compendial or validated procedure. A general chromatography chapter does not establish all reagent-to-analyte mappings.
Procurement note: Supplier formulations vary. Purchase the defined reaction system rather than substituting an indicator by name alone; bulk dry DNPH is not a routine substitute for a stabilized reagent.
Procurement links open the manufacturer's or distributor's page in a new tab. STAINIC does not sell these items; always buy products validated for diagnostic use and follow local regulations.